Get list of application layer protocols only

I'm currently using this to get a list of protocols found in a pcap file.

tshark -r ~/Downloads/smallFlows.pcap -Tfields -eframe.protocols | sort | uniq

I would like to narrow this down to application layer protocols only. Can I do this without filtering the output with a script (ie: Tshark and terminal commands only?)

EDIT

I managed to get it doing this:

tshark -r ~/Downloads/smallFlows.pcap -Tfields -eframe.protocols -R "tcp && ip.addr==192.168.3.131" -2 | sort | uniq | cut -d ':' -f 5

I'm curious to know if there's a better way of doing this though!