trouble shoot capture

Hello,

I am having an issue in running our in house developed application. This application access remotely setup SQL Server to establish connection. we were using the application since last 4 months. unfortunately on last friday i do not know what happened and just this location is not able to run the application as it is not able to establish connection with SQL server. As trouble shooting i have replaced router, by pass whole network just connected my laptop and tried to run application but did not worked.

So i connected my pc directly to ISP modem ( Charter) and captured the data flow between my pc and server. I am a new person for networking and do not know how to read the captured packets. so i am attaching the captured packet log with this.

please if you can make out of this log and understand what it is saying please advise me.

Thanks for looking into my question.